Fig. 4
From: Loss of MBNL1-mediated retrograde BDNF signaling in the myotonic dystrophy brain
Impaired retrograde transport of BDNF-TrkB signaling in the DM1 mouse brain. (a) Expression levels of DYNLL2 and p-TrkB in the EpA960/CaMKII-Cre and control mouse brains. The control group includes two non-Tg and one EpA960 animals. Relative amounts of DYNLL2 and p-TrkB are normalized according to GAPDH and TrkB, respectively. (b) Representative TrkB immunoreactivity of cortical-layer V neurons in EpA960/CaMKII-Cre mice at age 6 months. Quantification of the intensity of TrkB immunoreactivity in the proximal neurite region. TrkB immunoreactivity in the cell body is indicated by arrowhead. V, layer V neurons. Number of animals (N) and number of neurons (n) in each group is indicated. Data are mean ± SEM. *p < 0.05, **p < 0.01 by Student’s t-test. Scale: 50 μm