Fig. 3
Histopathological findings of the 33 years old vastus laterlais muscle biopsy. A Hematoxylin & Eosin, showing fiber size variability, fibers with multiple internalized nuclei and a slight accumulation of endomysial fibrotic tissue. B Gömöri trichrome staining showing increased endomysial tissue and multiple internalizations. C NADH-TR showing homogenous intermyofibrillary network. D ATPase 9.4 showing type 1 fibers predominance. E Distribution of fiber size of P1 showing global fibres hypotrophy compared to a control. F MyHC I and IIA immunofluorescence. G Histogram representing the total number of type 1 and type 2a fibers compared to a control (76.73%). H Pax7, Ki67, Laminin, Dapi immunofluorescence. Pax7 positive cells are indicated by pink arrows. Pax7/Ki67 positive cell, indicated by yellow square. I Histogram presenting the total number of Pax7/mm2 positive cells, showing a slight increase in the number of SCs compared to control. J Histogram showing the percentage of Pax7/Ki67 double positive cells on the total of Pax7 positive cells