Fig. 4

Transcriptomic analysis and expression of adhesion molecules in microglia and BAMs following genetic ablation of Igf1r. A Volcano plot and B heatmap showing the differential expression analysis of microglia from IGF-1R^{KO−tdTomato} and IGF-1R^{WT−tdTomato} mice sorted from brain. C Volcano plot and D heatmap showing the differential expression analysis of BAMs sorted from the brain of IGF-1R^{KO−tdTomato} and IGF-1R^{WT−tdTomato} mice E Functional enrichment analysis of BAMs from IGF-1R^{KO−tdTomato} and IGF-1R^{WT−tdTomato} mice using the Biological Process pathways from the Gene Ontology database, above: pathways upregulated in IGF-1R^{KO−tdTomato} mice, below: pathways downregulated in IGF-1R^{KO−tdTomato} mice. F–H Flow cytometry analysis of expression of α4 (CD49d), αL (CD11a) and β2 (CD18) integrins and PSGL-1 in BAMs, microglia and splenic macrophages from IGF-1R^{KO−tdTomato} (n = 3) and IGF-1R^{WT−tdTomato} (n = 3) mice. Data represented as mean ± SEM. Statistical analysis was performed by using unpaired t test with Welch´s correction, *p < 0.05: BAMs (α4: p = 0.757; β2: p = 0.629, PSGL-1: p = 0.034), Microglia (α4: p = 0.591; β2: p = 0.466, PSGL-1: p = 0.907), splenic macrophages (α4: p = 0.539; αL: p = 0.281; β2: p = 0.629, PSGL-1: p = 0.402)