Fig. 1

Characterization of IGF-1R^KO mouse model. A Diagram illustrating the genetic make-up of the CX3CR1-cre^ERT2 x IGF-1R floxed x Ai14 mouse model. B Gating strategy illustrating the sorting of tdTomato⁺ CD45 + CD11b + myeloid cells from the CNS of tamoxifen-treated IGF-1R^{KO−tdTomato} and IGF1R^{WT−tdTomato} mice. C DNA from sorted cells was tested by PCR using specific primers identifying the WT (1′100 bp), floxed (1′200 bp) or recombined (600 bp) Igf1r locus (n = 2). D Representative confocal pictures and E quantification of IGF-1R expression in Tomato + cells following staining with IGF-1R-specific antibody in CNS sections from IGF-1R^{KO−tdTomato} (n = 3) and IGF-1R^{WT−tdTomato} mice (n = 4): unpaired T-test with Welch´s correction, p < 0.0001 (microglia), p < 0.001 (meningeal BAMs) and Mann Whitney U test p = 0.0048 (perivascular BAMS) F) Bar graph shows difference in weight before and after tamoxifen treatment measured in IGF-1R^{KO−tdTomato} (n = 11) and IGF-1R^{WT−tdTomato} mice (n = 11) from 3 different experiments (Mann Whitney U test, p = 0.031). All values are presented as mean ± SEM. Asterisks indicate significant differences (∗ p < 0.05, ∗  ∗ p < 0.01 and ∗  ∗  ∗ p < 0.001, ∗  ∗  ∗  ∗ p < 0.0001)