Fig. 5

Electrophysiological characterization of pyramidal neurons in the PFC of CrT^+/y (WT) and CrT^−/y (KO) mice. Recordings were obtained from layer II/III pyramidal neurons from CrT^+/y (n = 8) and CrT^−/y (n = 5) animals at PND35-40, dots represent individual cells. a Representative traces (top) and quantification (bottom) of spontaneous excitatory postsynaptic currents (sEPSCs) in pyramidal cells of CrT^+/y and CrT^−/y animals. No differences were found between the genotypes in frequency (t-test, p = 0.477, n = 19 cells for CrT^+/y and 17 for CrT^−/y) and amplitude (Mann–Whitney test, p = 0.851, n = 19 cells for CrT^+/y and 17 for CrT^−/y). b Representative traces (top) and quantification (bottom) of firing frequency, showing an increased firing in CrT^−/y pyramidal neurons (t-test, p < 0.05, n = 23 cells for CrT^+/y and 19 for CrT^−/y). c CrT^−/y pyramidal neurons display higher membrane resistance (left, Mann–Whitney test, p < 0.05, n = 29 cells for CrT^+/y and 22 for CrT^−/y) and lower rheobase (right, t-test, p < 0.05, n = 23 cells for CrT^+/y and 19 for CrT^−/y) compared to controls. d Frequency vs. current plot showing persistently increased firing frequency in CrT^−/y cells across a range of injected currents (two-way RM ANOVA followed by Fisher’s LSD test, n = 23 cells for CrT^+/y and 19 for CrT^−/y). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data are expressed as mean ± SEM