Fig. 2

SnRNA-seq analysis of the cerebral cortex of CrT^+/y (WT) and CrT^−/y (KO) animals. a UMAP plot showing the populations identified in the snRNA-seq dataset of CrT^+/y (n = 2) and CrT^−/y (n = 2) animals. b Dot plot showing expression levels and gene detection of markers for the 7 major populations identified. c Bar graphs showing comparable distribution of each cell population between replicates and genotypes. d UMAP plot showing the populations identified in the snRNA-seq dataset of CrT^+/y and CrT^−/y animals by sample of origin. e Volcano plots showing the DEGs for each major cell population. The up- and downregulated genes are marked in red and blue, respectively, and their number is indicated at the top of each plot. Significant differences were detected in excitatory and inhibitory neurons, oligodendrocytes, and microglia, but not in astrocytes and vascular/endothelial cells (Wilcoxon Rank Sum test, adj p-value < 0.1)