Fig. 1

Presynaptic microenvironments are more susceptible to partial GLT-1 inhibition than postsynaptic microenvironments. Schematic showing presynaptic (A) and postsynaptic B expression of iGluSnFR. Two-photon microscopy images with iGluSnFR expression obtained in CA1 showing presynaptic iGluSnFR expression with punctate expression pattern (C), or postsynaptic iGluSnFR expression of a single dendritic segment (D). E Evoked iGluSnFR responses (5 pulses, 100 Hz) in CA1 stratum radiatum with and without DHK application. Shown are traces for pre and postsynaptic microenvironments. F DHK application increases iGluSnFR decay tau to a greater extent at presynaptic compared to postsynaptic membranes. Horizontal lines above iGluSnFR traces indicate the timing and duration of electrical stimulation. Scale bars in C-D: 10 µm. Scale bars in E: 25%ΔF/F, 100 ms. DHK (2 µM) traces scaled to match the peak of DHK (0 µM traces). Error bars indicate s.e.m. *** p < 0.001