Fig. 3

Sub-passage from OH inoculated mice cause disease with traits consistent with that from the original BH subtype inocula. A Disease duration of second pass mice inoculated with MV-BH (ms) (dashed lines) or MV-OH (ms) (solid lines) homogenates. B RT-QuIC curves displayed as an average with S.D. of all mice tested for each group (see Table 4 for number of replicate brains tested). Colored curves correspond with the groups in panel a. C Western blots showing proteinase K resistant PrP in the brains of sub-passaged mice inoculated with MV-BH (ms) (left panel) or MV-OH (ms) (right panel) homogenate. To account for differences in PrP^Res signal due to the different deposition patterns between MV1 and MV2 inoculation groups, samples were loaded at different amounts to allow for better comparison of banding patterns. Numbers to the left indicate molecular weight in kDa. D. Lesion profile comparisons of MV-BH (ms) (solid line) and MV-OH (ms) (dashed line) inoculated mice are shown for MV1 (top panel) and MV2 (bottom panel) groups as well as young uninoculated mice (177 dpi; bottom panel, gray) controls. Shown are the mean and S.D. Mann–Whitney U test results comparing lesion scores for each brain region and each inoculum can be found in Additional File 4: Table S1B. See Table 4 for number of replicates tested for each panel