Fig. 5
From: CIC reduces xCT/SLC7A11 expression and glutamate release in glioma
Effect of CIC overexpression or NMDA receptor antagonist on glioma-conditioned media-mediated neuronal toxicity. A Glutamate and 2-HG levels in culture media incubated with TS603 Ctrl or CIC line. B Schematic of conditioned media (CM) using TS603 lines in NeuroCult media for 72 h. The figure was created with BioRender.com. C PC12 cell viability in the CM harvested from TS603 cells transduced with Ctrl or CIC. D PC12 cell viability in response to glutamate treatment with the indicated concentrations. E PC12 cell viability in the CM with or without MK801 (NMDA receptor antagonist) as assessed by MTT assay. The absorbance value is normalized to value obtained from NeuroCult CM. Mean ± SEM of at least three independent experiments. *p < 0.05, **p < 0.01