Fig. 2
From: CIC reduces xCT/SLC7A11 expression and glutamate release in glioma
Effect of overexpression or knockout of CIC on glioma proliferation and its target genes. A, B TS603 tumorspheres were transduced with Ctrl or CIC as determined by immunoblot (A) and immunostaining (B) Scale bar = 40 μm. C TS667 tumorspheres were transduced with lentiCRISPR v2-puro (Ctrl) or lentiCRISPR v2-sgCIC1/2-puro, and CIC protein levels were assessed by immunoblot. D, E Cell proliferation rate of CIC-proficient and CIC-deficient glioma lines as assessed by MTT assay. F Colony formation of TS603 expressing Ctrl or CIC in soft agar. G, H The expression of CIC target genes in CIC-proficient and CIC-deficient glioma lines as determined by qPCR (n = 3). Mean ± SED of at least three independent experiments