Fig. 9

Ctgf production upon nerve injury is H₂O₂- and YAP-dependent. A YAP (red) expression and localization in SCs (expressing GFP under the plp-promoter, green) in control nerves and 3/4 days post-crush, in the absence or presence of catalase delivered into the sciatic nerve. NF is in white and nuclei are stained with DAPI (blue). Asterisks mark YAP-positive SCs. Scale bars: 20 µm. A magnification of YAP localization in control nerves in shown in panel B. Scale bar: 10 µm. C, D Representative immunostaining of YAP and Ctgf in primary SCs exposed to 50 µM H₂O₂ w/wo the pan-TEAD autopalmitoylation inhibitor VT107 (C). YAP is in green, Ctgf in red and nuclei are in blue. Scale bars: 50 µm. Quantification of Ctgf levels by ELISA is shown in D. N = 3, *p = 0.0166, ns = not significant