Fig. 4

Ontological analysis of the proteins differentially expressed between WT and cNEB KO and proteomic differences induced following piperine administration. A Ontological associations between 111 proteins upregulated in cNEB KO (blue) and 139 proteins upregulated in WT mice (red) were established using Metascape and visualised using Cytoscape. Grey lines indicate a direct interaction, circle size is determined by enrichment and circle colour is determined by p value. Proteins upregulated in the WT mice can also be considered as down regulated in the cNEB KO. WT and cNEB KO networks were created separately with identical enrichment and p value scaling parameters. For graphical representation both WT and cNEB KO networks were scaled to match the enrichment key. The clusters with the highest enrichment are documented in the table shown in B, with the enrichment, p value and proteins present within the cluster. All full list of all clusters and proteins present is available in Additional file 1: Table S2 (B). C Venn diagram depicting detected proteins that were significantly expressed in control (purple) and following piperine administration (green), as well as those unchanged between experimental groups. Bar graphs depicting significant differences present in the three proteins which possessed significant differences between either WT and cNEB KO or between Control and Piperine administration, the two datasets were not subjected to comparative statistics. D Pie charts represent the Uniprot binding terms associated with the significantly up regulated proteins in either control or piperine administration obtained using DAVID bioinformatics database