Fig. 9
From: Bona fide atypical scrapie faithfully reproduced for the first time in a rodent model
Western blotting comparison of a classical scrapie isolate (SSBP/1) and the prions extracted from spontaneous-diseased TgShI112 or atypical scrapie inoculated TgVole (4x) and TgVole (1x) mouse brains after digestion with proteinase K (PK). Electrophoretic migration profiles of prions from TgVole (1 × and 4x, with respect to mouse PrP expression levels) inoculated intracerebrally with ShTgSPON are similar and characterized by the presence of a 7–10 kDa band, also indistinguishable from ovine atypical scrapie isolate inoculated in the same models. Both models challenged with ShTgSPON show the characteristic 7–10 kDa fragment indistinguishable from atypical scrapie, indicating the spontaneous formation of an atypical ovine prion in TgSh112I model. Anti PrP antibody 12B2, 1:5000. MW: Molecular weight