Fig. 8
From: Bona fide atypical scrapie faithfully reproduced for the first time in a rodent model
Bank vole bioassay. a PET blot detection of protease resistant PrPSc in coronal sections of the brain, representing telencephalon (1), diencephalon (2), midbrain (3), and hindbrain (4) from representative Bv109I. Isolates indicated on the Left. PrPSc was detected with mAb 6C2. b Representative replica Western blots showing PK-treated (PK) PrPSc from the brains of voles inoculated with atypical scrapie (AS, n = 9), ShTgSPON (n = 12) and classical scrapie (SSBP/1, n = 9), as indicated on the Top of the blots, and analyzed with mAbs recognizing different epitopes on PrP, as indicated on the Left of the blots (SAF84 epitope at 167 to 173; 12B2 epitope at 89 to 93). Notice absence of labelling for the c-terminal epitope for both AS and ShTgSPON. c Lesion profiles in groups of Bv109I infected with atypical scrapie (AS), ShTgSPON and classical scrapie (SSBP/1). Data points represent the mean ± SEM of at least five voles per group. Brain-scoring areas: medulla (1), cerebellum (2), superior colliculus (3), hypothalamus (4), thalamus (5), hippocampus (6), septum (7), retrosplenial and adjacent motor cortex (8), and cingulate and adjacent motor cortex (9)