Fig. 3

Functional significance of the interaction between spastin and CHMP2B^Intron5 in a mouse model of FTD3. a Western blot analysis of the effect of CHMP2B^Intron5 on spastin in a mouse model of FTD caused by mutant CHMP2B. The double bands are presumably two isoforms of spastin and the lower band corresponds to the M87 isoform that is used for transfection experiments throughout this study. b Level of total soluble spastin in the cortex of tTA:CHMP2B^Intron5 mice. Values are mean ± SEM from three western blot experiments. *p < 0.05, **p < 0.01 by two-sided t test. c Representative images from identical areas of the cortex double-stained for p62 (red) and spastin (green). P62 is known to co-aggregate with CHMP2B^Intron5 that is specifically expressed in excitatory neurons in this mouse model. The squares indicate areas shown at higher magnification in the adjacent panels. Scale bar: 20 µm. d Fraction of spastin signal overlapping with p62, calculated with Mander’s overlap coefficient. The analysis was done with the JACop plugin in Image J. *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA and Bonferroni post hoc test for multiple comparisons