Fig. 1

Increased biochemical interaction between spastin and CHMP2B^Intron5. a Diagram of CHMP2B^WT and CHMP2B^Intron5. CC: coiled coil. MIM: Microtubule Interaction Motif. b Proteins that coimmunoprecipitated with FLAG antibody were analyzed by western blots with spastin antibody. The experiment was repeated 4 times. After normalizing against the relative abundance of CHMP2B^WT versus CHMP2B^Intron5, 11 times more spastin was bound to CHMP2B^Intron5 than CHMP2B^WT. c Immunoprecipitation with spastin antibody followed by western blot analysis with FLAG antibody. d and e Co-immunostaining analysis shows colocalization of p62 d and endogenous spastin e with CHMP2B^Intron5 aggregates