Fig. 4

Astrocytic aSyn accumulations are free of the canonical aSyn aggregation markers. a-c The GFAP-positive astrocytic accumulations in DLB1 cingulate cortex were negative to aSyn pS129 (AB EP1536Y), ubiquitin and p62. Images of cortical LBs are included as positive controls for aSyn pS129, ubiquitin and p62 reactivity. Images for Fig. 4a, c taken using Olympus slide scanner at 40 × magnification. d The astrocytic aSyn signal (arrows) was largely abolished after PK treatment in DLB cingulate cortex. The 2F10-E12 staining was included as a positive control to show the PK resistance of LBs and LNs. Images taken from the cortical deep grey matter (layers V–VI) DLB1 cingulate cortex. e The star-shaped astrocytic aSyn accumulations were revealed by the 5G4 antibody, but not by the SYNO4 antibody in the DLB cingulate cortex. Representative images from the cortical deep grey matter (layers V–VI) of DLB1 and DLB3 cingulate cortex. agg-aSyn = aggregated alpha-synuclein; aSyn = alpha-synuclein; DLB = dementia with Lewy bodies; GFAP = glial fibrillary acidic protein; LB = Lewy body; LN = Lewy neurite; PK = proteinase K