Fig. 5

Humanized anti-sCD146 antibody mucizumab significantly decreases U87 cell proliferation, migration and invasion and hampers CSC and EMT in vitro. U87 cells were treated with conditioned media (CM) containing Irrelevant IgG, bevacizumab (Avastin), Mucizumab, or combination of both antibodies for proliferation (a), migration (b), and invasion assays (c). EMT and CSC markers were also examined at the mRNA (d and e) and protein (f and g) levels. Average of 3 experiments is shown; *p < 0.05, **p < 0.01, ***p < 0.001, experimental vs control