Table 2 List of antibodies used
From: Müller cell degeneration and microglial dysfunction in the Alzheimer’s retina
Antigen | Antibody (catalog no.) | Dilution | Source |
|---|---|---|---|
Primary antibodies | |||
Aβ | Monoclonal Mouse Anti-Human Beta-Amyloid Clone 6F/3D (M0872) | 1:100 | Agilent/Dako |
TUBB3 | Purified rabbit anti-Tubulin P 3 (845501) | 1:500 | Biolegend |
GFAP | Polyclonal rabbit anti-Glial Fibrillary Acidic Protein antibody (Z0334) | 1:300 | Agilent/Dako |
GS | Purified Monoclonal Mouse Anti-Glutamine Synthetase Antibody, clone GS-6 | 1:300 | MilliporeSigma |
IBA-1 | Polyclonal rabbit Anti-IBA-1 antibody (019–19741) | 1:500 | Wako |
12F4 | Purified mouse Anti-β- Amyloid, 1–42 Clone 12F4 (805504) | 1:100 | Biolegend |
6E10 | Purified mouse Anti-β-Amyloid, 1–16 Clone 6E10 (803001) | 1:100 | Biolegend |
Secondary antibodies | |||
Cy3 | Goat Anti-mouse Cy3 Alexa 546 IgG1 secondary antibody (A21123) | 1:400 | Fisher scientific |
FITC | Goat Anti-rabbit Alexa 488 secondary antibody (A11070) | 1:500 | Fisher scientific |