Fig. 5
From: Müller cell degeneration and microglial dysfunction in the Alzheimer’s retina
Thresholding for GS and GFAP immunostaining. Glutamine synthetase (GS) immunolabelling (red) marks both resting and activated Müller cells. Glial filamentary acidic protein (GFAP) immunolabelling (green) is present in activated Müller cells and astrocytes. Representative images of cross-sections stained with GS and GFAP. A, D Pixels positive for both GS and GFAP (yellow) activated Müller cells. B, E Pixels positive for GS Only represent resting Müller cells. C, F Pixels positive for GFAP Only represent astrocytes