Fig. 2

Comparisons of immunofluorescence using three Aβ antibodies. Cross sections of Alzheimer’s disease (AD) retina were processed with three monoclonal mouse antibodies against Aβ: A, E Clone 6F/3D, which labels β-amyloid containing the N-terminal epitope (Agilent, CA, USA); B, F 12F4, which labels the C-terminus of β-amyloid and is specific for the isoform ending at the 42nd amino acid (Biolegend, CA, USA); and C, G 6E10, which recognizes the epitope that lies within the amino acids 3–8 of β-amyloid as well as the precursor forms (Biolegend, CA, USA). Note that immunolabeling pattern is consistent with all three antibodies and identifies what is likely intracellular labelling of retinal ganglion cells (asterisks) and extracellular deposits (arrowheads). Negative control sections in which the primary antibody was omitted resulted in no immunofluorescence (D). All sections were also imaged under 488 nm for autofluorescence that may be associated with melanopsin containing retinal ganglion cells. Note that no green or yellow/orange signals were observed in A–D in which both 543 nm and 488 nm were used, and confirmed in E–H in which the same section was imaged under 488 nm only. I-L) Cross sections of control retina were also processed for all three monoclonal mouse antibodies against Aβ and demonstrated both what is likely intracellular labelling of retinal ganglion cells (asterisks) and extracellular deposits (arrowheads). DAPI was used to label nuclei throughout all panels and imaged under 405 nm