Fig. 7
From: Protein citrullination marks myelin protein aggregation and disease progression in mouse ALS models
Citrullinated proteins are enriched in insoluble protein aggregates. A, B Sedimentation assay for citrullinated proteins in lumbar spinal cord. Homogenates from different disease stages and age-matched controls were centrifuged. Proteins in the supernatant and the pellet were resolved by SDS-PAGE. Citrullinated proteins were detected by the ACM method. C, D Filter trap assay for citrullinated protein aggregates from the spinal cord. E, F Quantification of optical density in C, D, respectively. G, H Filter trap assay for ubiquitinated protein aggregates. I, J Quantification of optical density in G, H, respectively. n = 4 in all groups. Statistics: unpaired t test for comparing transgenic mice with their age matched controls. *p < 0.05; **p < 0.01; ***p < 0.001