Fig. 3
From: Protein citrullination marks myelin protein aggregation and disease progression in mouse ALS models
PAD2, but not PAD3, PAD4 and PAD1 expression, are increased. A, B Levels of PAD2 mRNA from lumber spinal cords of SOD1G93A and PFN1C71G mice at different disease stages and their age-matched nTg controls. C, D Western blots and quantification of PAD2 protein levels from lumber spinal cords of SOD1G93A and PFN1C71G mice at different disease stages and their age-matched nTg controls. E Comparison of PAD2 protein levels in the spinal cords of SOD1G93A mice at paralysis stage with the age-matched nTg and SOD1 wild type (WT) transgenic mice. F Same as E but comparing PFN1C71G, PFN1WT, and nTg mice. Note that the endogenous SOD1 in the nTg mice was below detection at the loading amount. G, H Western blot and quantification for PAD3 and PAD4 in SOD1G93A and PFN1C71G mice, respectively. I, J PAD1 mRNA levels in SOD1G93A and PFN1C71G mice, respectively. For quantification, n = 4 in all groups. Statistics: unpaired t test. *p < 0.05; **p < 0.01. Unmarked pairs, or n.s., are not significantly different