Fig. 2
From: Protein citrullination marks myelin protein aggregation and disease progression in mouse ALS models
PC increases progressively in astrocytes but decreases in neurons in the spinal cord. A Double immunofluorescence staining for GFAP and citrullinated proteins in ventral horn in SOD1G93A mice. B, C Quantification of fluorescent intensity of GFAP and citrullinated proteins, respectively, in A. D Double immunofluorescence staining for GFAP and citrullinated proteins in ventral horn in PFN1C71G mice. E, F Quantification of fluorescent intensity of GFAP and citrullinated proteins, respectively, in D. G Double immunofluorescence staining for NeuN and citrullinated proteins in ventral horn in SOD1G93A mice. H Quantification of fluorescent intensity of citrullinated proteins in NeuN-positive nuclei in G. I Double immunofluorescence staining for NeuN and citrullinated proteins in ventral horn in PFN1C71G mice. J Quantification of fluorescent intensity of citrullinated proteins in NeuN-positive nuclei in I. The nTg mice used as controls for mutant SOD1 mice were ~ 150 days old. The nTg mice used as controls for mutant PFN1 mice were ~ 220 days old. For quantification, n = 8 in all groups. Statistics: one-way ANOVA with Bonferroni post-hoc test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Unmarked pairs are not significantly different. Arrows point to examples of NeuN-positive and citrullinated protein-positive cells. Note the panels for citrullinated protein staining in G, I were overexposed in order to visualize the weak signals in neurons in paralysis stage of the disease