In vitro and in vivo differences in neurovirulence between D614G, Delta And Omicron BA.1 SARS-CoV-2 variants

Table 1 List of differentiation media

Name	Reagents with final concentration	Manufacturer
hiPSC medium	Stemflex medium	ThermoFisher Scientific
	100 IU/ml penicillin	Lonza
	100 µg/ml streptomycin	Lonza
	10 µl/ml fresh RevitaCell	ThermoFisher Scientific
Differentiation medium	Advanced DMEM/F12 medium	ThermoFisher Scientific
	100 IU/ml penicillin	Lonza
	100 µg/ml streptomycin	Lonza
	0.1 mM non-essential amino acids	Lonza
	1% N2 supplement	ThermoFisher Scientific
	10 ng/ml fresh Human Recombinant Neurotrophin-3 (NT3)	Stemcell Technologies
	10 ng/ml fresh brain-derived neurotrophic factor (BDNF)	Prospecbio
	200 ng/ml fresh laminin	Corning
	4 µg/ml fresh Doxycycline	Sigma
Ngn2 medium	Neurobasal medium	ThermoFisher Scientific
	100 IU/ml penicillin	Lonza
	100 µg/ml streptomycin	Lonza
	2 mM glutamine	Lonza
	2% B27 minus RA supplement	ThermoFisher Scientific
	10 ng/ml fresh Human Recombinant Neurotrophin-3 (NT3)	Stemcell Technologies
	10 ng/ml fresh brain-derived neurotrophic factor (BDNF)	Prospecbio
	4 µg/ml fresh Doxycycline (DOX)	Sigma

ISSN: 2051-5960