Name | Reagents with final concentration | Manufacturer |
---|---|---|
hiPSC medium | Stemflex medium | ThermoFisher Scientific |
100Â IU/ml penicillin | Lonza | |
100 µg/ml streptomycin | Lonza | |
10 µl/ml fresh RevitaCell | ThermoFisher Scientific | |
Differentiation medium | Advanced DMEM/F12 medium | ThermoFisher Scientific |
100Â IU/ml penicillin | Lonza | |
100 µg/ml streptomycin | Lonza | |
0.1Â mM non-essential amino acids | Lonza | |
1% N2 supplement | ThermoFisher Scientific | |
10Â ng/ml fresh Human Recombinant Neurotrophin-3 (NT3) | Stemcell Technologies | |
10Â ng/ml fresh brain-derived neurotrophic factor (BDNF) | Prospecbio | |
200Â ng/ml fresh laminin | Corning | |
4 µg/ml fresh Doxycycline | Sigma | |
Ngn2 medium | Neurobasal medium | ThermoFisher Scientific |
100Â IU/ml penicillin | Lonza | |
100 µg/ml streptomycin | Lonza | |
2Â mM glutamine | Lonza | |
2% B27 minus RA supplement | ThermoFisher Scientific | |
10Â ng/ml fresh Human Recombinant Neurotrophin-3 (NT3) | Stemcell Technologies | |
10Â ng/ml fresh brain-derived neurotrophic factor (BDNF) | Prospecbio | |
4 µg/ml fresh Doxycycline (DOX) | Sigma |