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Table 1 List of differentiation media

From: In vitro and in vivo differences in neurovirulence between D614G, Delta And Omicron BA.1 SARS-CoV-2 variants

Name Reagents with final concentration Manufacturer
hiPSC medium Stemflex medium ThermoFisher Scientific
100 IU/ml penicillin Lonza
100 µg/ml streptomycin Lonza
10 µl/ml fresh RevitaCell ThermoFisher Scientific
Differentiation medium Advanced DMEM/F12 medium ThermoFisher Scientific
100 IU/ml penicillin Lonza
100 µg/ml streptomycin Lonza
0.1 mM non-essential amino acids Lonza
1% N2 supplement ThermoFisher Scientific
10 ng/ml fresh Human Recombinant Neurotrophin-3 (NT3) Stemcell Technologies
10 ng/ml fresh brain-derived neurotrophic factor (BDNF) Prospecbio
200 ng/ml fresh laminin Corning
4 µg/ml fresh Doxycycline Sigma
Ngn2 medium Neurobasal medium ThermoFisher Scientific
100 IU/ml penicillin Lonza
100 µg/ml streptomycin Lonza
2 mM glutamine Lonza
2% B27 minus RA supplement ThermoFisher Scientific
10 ng/ml fresh Human Recombinant Neurotrophin-3 (NT3) Stemcell Technologies
10 ng/ml fresh brain-derived neurotrophic factor (BDNF) Prospecbio
4 µg/ml fresh Doxycycline (DOX) Sigma