Fig. 6

Drug screening in vitro. A, B Cell toxicity assays on CD105⁺ cells lines. Different concentrations of temozolomide (A) and bevacizumab (B) on MGMT promoter methylated CD105⁺ cell line (GBM B16) and MGMT wildtype CD105⁺ cell line (GBM B14) cultured for 96h. Fluorescence units represent the cell viability detected at 24 h, 48 h, 72 h and 96 h. Control cells were kept in culture medium without adding any drug. C Heatmap showing the toxicity of 88 clinical compounds (10μM) against 3 GBM CD105⁺ cell lines and U87 cell line. Cell toxicities are displayed by the range of the colors from red to blue as high to low. The rows are clustered using correlation distance. D Cell toxicity assay showing the effect of different concentrations of Doxorubicin, Idarubicin HCl, Fludara and ABT-751 on CD105⁺ and U87 cells. Cell viability was detected at 48 h and 96 h. **P < 0.01. E Interaction plot between drugs and genes. Each circle represents a gene, and its area symbolizes relevance. F Kaplan–Meier curves showing the relation between patient survival and drug-interacted genes as analyzed using patient data from TCGA database. P value is calculated by logrank test. *P < 0.05