Fig. 5
From: BDNF-dependent modulation of axonal transport is selectively impaired in ALS
Truncated TrkB and p75NTR levels are increased in SOD1G93A muscles. Representative immunoblots for A BDNF, B TrkB.FL and truncated TrkB, and C p75NTR, comparing wild-type (WT) and SOD1G93A tibialis anterior (TA) and soleus (Sol) muscles. Control tissue (Ctrl) from age-matched WT spinal cord (P94) was included. The difference in migration patterns suggests that TrkB.FL, but not truncated TrkB, undergoes distinct post-translational modifications in skeletal muscles. Immunoblot quantifications: D BDNF (genotype p = 0.95, muscle ***p < 0.001, interaction p = 0.08); E TrkB.FL (genotype p = 0.476, muscle p = 0.342, interaction p = 0.507); F truncated TrkB (genotype ***p < 0.001, muscle p = 0.239, interaction p = 0.384); and G p75NTR (genotype **p = 0.008, muscle p = 0.932, interaction p = 0.14) (n = 10). Data were compared by two-way ANOVA and Holm-Å Ãdák's multiple comparisons tests. *p < 0.05, **p < 0.01, ***p < 0.001. Means ± SEM are plotted for all graphs. Black (P73) and grey (P94) circle borders indicate age-matched mice