Fig. 3
From: BDNF-dependent modulation of axonal transport is selectively impaired in ALS
BDNF stimulation enhances axonal transport of signalling endosomes in primary embryonic ventral horn neurons from WT, but not SOD1G93A mice. A Schematic of primary embryonic ventral horn cultures plated in microfluidic chambers (MFCs) with or without 50 ng/µl of BDNF added to both somatic and axonal compartments. B Speed distribution curves of signalling endosome transport in WT and SOD1G93A primary ventral horn neurons in MFCs. C Axonal transport dynamics of the same cultures upon addition of 50 ng/ml of BDNF, with the mean endosome speeds shown in D (WT vs. SOD1G93A: p = 0.068; WT −/ + BDNF: *p = 0.041; SOD1G93A −/ + BDNF: p = 0.735; WT vs. SOD1G93A + BDNF: *p = 0.037). E Mean speeds in individual motor neuron axons (WT vs. SOD1G93A: p = 0.7308; WT −/ + BDNF: ***p < 0.001; SOD1G93A -/ + BDNF: p = 0.576; WT vs. SOD1G93A + BDNF: ***p < 0.001). F Speed of individual HCT-555-positive signalling endosomes (white circles; black lines represent the median and the dashed lines represent the upper and lower quartiles). G Percentage of signalling endosomes pausing (WT vs. SOD1G93A: p = 0.122; WT −/ + BDNF: p = 0.745; SOD1G93A −/ + BDNF: p = 0.154; WT vs. SOD1.G93A + BDNF: p = 0.791). Statistical analyses were performed using unpaired, two-tailed t-tests (n = 3 biological replicates). See also Additional file 2: Table S2