Fig. 1
From: BDNF-dependent modulation of axonal transport is selectively impaired in ALS
In vivo axonal transport dynamics of signalling endosomes are similar in motor neurons innervating fast and slow muscles in wild-type mice. A Time-lapse images of retrogradely transported (i.e., right-to-left), HCT-555-containing signalling endosomes from a single sciatic nerve axon. Yellow and cyan arrowheads indicate retrogradely moving signalling endosomes, while red arrowheads point to a paused endosome. Frame rate = 3.1 frame/s; scale bar = 10 µm. B Endosome frame-to-frame speed distribution curves in motor axons innervating tibialis anterior (TA), lateral gastrocnemius (LG) and soleus muscles in basal conditions. C Speed of individual HCT-555-positive signalling endosomes (white circles) in motor axons innervating TA, LG, and soleus. Black line represents the median and the dashed lines represent the upper and lower quartiles. No statistical comparisons were performed on these datasets due to overpowering. D Mean speeds of HCT-555-positive signalling endosomes per individual motor neuron axons innervating TA, LG, and soleus (p = 0.135, one-way ANOVA, n = 16–21). E Average (triangles) and maximum (circles) endosome speeds per animal in motor axons innervating TA, LG, and soleus (average: p = 0.193; maximum: p = 0.715; one-way ANOVA, n = 5–7). F Relative percentage of time signalling endosomes paused per animal in axons innervating the TA, LG, and soleus (**p = 0.002, one-way ANOVA, n = 5–7). The colour coding of individual datapoints is consistent within the muscle type and reflects the same animal in Fig. 1D, 1E and 1F. *p < 0.05, Kruskal–Wallis multiple comparisons test. See also Additional file 2: Table S1