Fig. 5

Measurements of the volume of different brain regions using MRI. Rats were scanned using MRI (n = 6 per group) to measure the volumes of different brain regions as the number of voxels in the ROI. A Representative horizontal, sagittal, and coronal T2-weighted RARE images are shown. 3D-reconstructed images were registered and segmented with a template image from the SIGMA rat brain atlas. The scale bar in each panel represents 5 mm. Representative ROIs used in volume measurements, such as hippocampi and LEC (blue—right; green—left), are highlighted on the three orthogonal slices and the 3D projection. Volume was measured in B the whole brain, C regions of the hippocampus and D regions of the EC. Data from the EC and the hippocampus were normalized to total brain volume. Data are presented as the means ± SEM. *p < 0.05. DG: dentate gyrus. CA cornu ammonis, MEC medial entorhinal cortex, LEC lateral entorhinal cortex, EC entorhinal cortex