Fig. 4

Axonal proteomic profiling before and immediately after injury. A Axonal and neuronal compartment fractions (A/N) in both control and injury treatments (C/I) were processed for LFQ mass spectrometry. PCA and heatmap of differentially expressed proteins (n = 2). B Volcano plots depicting the quantitative protein changes following injury compared with control treatment for the axonal and the neuronal fractions. C Total number of proteins present in the neuronal and axonal fractions (upper panel). Human Protein Atlas top terms of the enrichment analysis for the neuronal fraction proteins (lower panel). D Top GO Biological Processes terms for the enrichment of the axonal (upper panel) or the neuronal fraction (lower panel). E Enrichment analysis of the axonal fraction proteins using as background the neuronal fraction proteins. Significant terms for each GO category were clustered by similarity and most representative words of the cluster’s terms assigned. See also Additional file 9: Fig. S3