Fig. 2

miRNA biogenesis components go awry in human HD striatum. Representative immunoblots of endogenous DGCR8, TRBP, Drosha, Dicer, Ago2 and Exportin in the soluble fraction of A the striatum or B the cortex of HD patients and Controls. See Methods for the list of antibodies. C–E Protein quantifications of each protein according to disease or brain region. Bar graphs with standard error of the mean (SEM) are shown, where the average of Controls is set as 100%. In all cases, the HD group is presented as pooled or per stage. Statistics: Ctl vs. HD as a group was calculated using a Mann–Whitney test. Ctl vs. HD stages was calculated using an analysis of covariance followed by the Kruskal–Wallis comparison test. Significant fold changes are provided for each group. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Abbreviations: Ctl, Controls; HD, Huntington’s disease; HD2, Vonsattel grade 2; HD3, Vonsattel grade 3; HD4, Vonsattel grade 4