Fig. 5

Images showing Calb immunostaining in NTD at 28 (A, B), 41 (C–F), and 174 (G–J) wk and in DS at 32 (K, L), 44 (M–P) and 196 (Q–T) wk. A small number of Calb-ir cells with pyramidal shape were visible in layer V in a 28 wk NTD (B) compared to extensive Calb-ir neuropil. Calb positive pyramidal cells in SG and IG layers at 32 wk in DS (K, L). At 41 weeks in NTD, Calb immunoreactivity was seen in layer I (C), in small cells in layers II/III (C, D) and fusiform neurons in layers V/VI (E, F). At 44 weeks in DS, Calb immunostaining was only observed in small cells in layers II/III (N) and V/VI (O, P). In the oldest cases (174 wk NTD and 196 wk DS), Calb-ir interneurons and pyramidal cells were seen in layer II (G, Q), while strong cytoplasmic Calb immunoreactivity was seen in pyramidal cells in layer III (H, J, R, T) and layer V (I, S). Inset in T shows a higher magnification image of Calb-ir interneuron in layer III at 196 weeks in DS. Note greater Calb immunoreactivity in the pyramidal apical process in the oldest NTD (H–J) compared to the oldest DS case (R–T). Scale bars: A–I = 50 µm; J, T and inset = 20 µm; K–S = 50 µm