Table 3 Details of antibodies used for confocal analysis
From: Oligodendrocyte lineage is severely affected in human alcohol-exposed foetuses
Antibodies | Reference | Purified species | Supplier | Dilution | Target | Solution of incubation |
|---|---|---|---|---|---|---|
GABA | A2052 | Rabbit | Sigma-Aldrich | 1/400 | Neurotransmitter | 1% BSA, 3% Triton X-100 in PBS |
GFAP | Ab10062 | Mouse | Abcam | 1/200 | Intermediate filament protein | 1% BSA, 3% Triton X-100 in PBS |
MAP2 | M4403 | Mouse | Sigma Aldrich | 1/100 | Brain microtubule-associated protein | 1% BSA, 3% Triton X-100 in PBS |
Olig2 | AF2418 | Rabbit | R&D System | 1/200 | Oligodendrocyte lineage transcription factor 2 | 1% BSA, 3% Triton X-100 in PBS |
PDGFRα | AF1062 | Goat | R&D System | 1/200 | Platelet derived growth factor subunit α | 1% BSA, 3% Triton X-100 in PBS |