Fig. 7

Number of γH2AX + cells was significantly increased in temporal pole and visual cortex of demented but not in resilient when compared to control brains (a). Representative photomicrographs of γH2AX + cells on sections from control, resilient and demented cases containing visual cortex (b). Representative immunofluorescence images of colocalization of γH2AX + cells (brown) with neurons (red) (7c 1–3, showing a progressively zoomed-in brain region), GFAP + astrocytes (green) (c 4–6, showing GFAP alone, 7c4, γH2AX alone, 7c5, both GFAP-γH2AX, 7c6), and IBA1 + microglia (green) (7c 7–9, showing IBA1 alone, 7c7, γH2AX alone, 7c8, both IBA1-γH2AX, 7c9). C Control (Braak 0-II); R Resilient (Braak III/IV); D Demented (Braak III/IV); **p < 0.01; ***p < 0.001. Scale bars 1 mm, 50 μm, and 20 μm. White arrows indicate colocalization between γH2AX+ cells and neurons, astrocytes, and microglia, respectively