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Table 1 Study 1—Immunohistochemistry of axons in Thy1-YFP-16 mice (Figs. 2 and 3). Student’s t-test or two-way ANOVA results with Holm-Sidak post hoc test and Cohen’s d effect size

From: Acute axon damage and demyelination are mitigated by 4-aminopyridine (4-AP) therapy after experimental traumatic brain injury

Figure 2- Outcome measures t, df or F (DFn, DFd); p-value Adjusted p-value Cohen’s d effect size
YFP particles t12 = 9.785; p < 0.0001
Interaction:
F1,10 = 0.098, p = 0.7607
Sex:
F1,10 = 0.022, p = 0.8852
Drug:
F1,10 = 78.30, p < 0.0001
Males:
TBI veh vs TBI 4-AP:
p = 0.0006
Females:
TBI veh vs TBI 4-AP:
p = 0.0002
4-AP: TBI males vs TBI females:
p = 0.9381
TBI veh vs TBI 4-AP:
d = 5.231
% Area of
YFP particles
t12 = 10.05; p < 0.0001
Interaction:
F1,10 = 1.205, p = 0.2980
Sex:
F1,10 = 0.00000275, p = 0.9987
Drug:
F1,10 = 98.41, p < 0.0001
Males:
TBI veh vs TBI 4-AP:
p = 0.0005
Females:
TBI veh vs TBI 4-AP:
p < 0.0001
4-AP: TBI males vs TBI females:
p = 0.7028
TBI veh vs TBI 4-AP:
d = 5.370
Figure 3- Outcome measures F (DFn, DFd); p-value Adjusted p-value Cohen’s d effect size
Caspr+ heminodes Interaction:
F1,12 = 17.21, p = 0.0013
Injury:
F1,12 = 61.00, p < 0.0001
Drug:
F1,12 = 16.80, p = 0.0015
sham veh vs TBI veh:
p < 0.0001
sham 4-AP vs TBI 4-AP:
ns, p = 0.0651
TBI veh vs TBI 4-AP:
p = 0.0003
sham veh vs TBI veh:
d = 5.591
sham 4-AP vs TBI 4-AP:
d = 1.917
TBI veh vs TBI 4-AP:
d = 3.713
Caspr length difference Interaction:
F1,12 = 15.66, p = 0.0019
Injury:
F1,12 = 31.46, p = 0.0001
Drug:
F1,12 = 15.66, p = 0.0019
sham veh vs TBI veh:
p = 0.0001
sham 4-AP vs TBI 4-AP:
ns, p = 0.6040
TBI veh vs TBI 4-AP:
p = 0.0005
sham veh vs TBI veh:
d = 5.340
sham 4-AP vs TBI 4-AP:
d = 0.754
TBI veh vs TBI 4-AP:
d = 3.302
% Atypical
Kv1.2 domains
Interaction:
F1,12 = 14.47, p = 0.0025
Injury:
F1,12 = 293.7, p < 0.0001
Drug:
F1,12 = 7.281, p = 0.0194
sham veh vs TBI veh:
p < 0.0001
sham 4-AP vs TBI 4-AP:
p < 0.0001
TBI veh vs TBI 4-AP:
p = 0.0012
sham veh vs TBI veh:
d = 11.136
sham 4-AP vs TBI 4-AP:
d = 6.312
TBI veh vs TBI 4-AP:
d = 5.511
  1. veh Saline vehicle control; ns Non-significant (p > 0.05). Sham veh vs sham 4-AP ns for each measure (p > 0.4 or higher). Veh TBI males vs veh TBI females ns for each measure (p > 0.7 or higher)