Fig. 2

HTT and p62 inclusion density follows an increasing caudal to rostral gradient in parasagittal neocortical regions of HD. a Macroscopic photographs of the formalin fixed right half brain of a 56-year-old man with adult-onset HD (CAG: 47/21) demonstrating the lateral and medial aspects. The brain (fresh weight: 981.6 g) appeared diffusely small. Vertical lines indicate the plane of section to obtain the coronal slices of images (b)–(d). b–d Select coronal slices of this brain from rostral to caudal with rectangles showing the Brodmann areas of interest, BA9 (superior frontal cortex, b), BA4 (motor cortex, c) and BA7/ BA17 (precuneal cortex and calcarine/visual cortex, respectively, d). e–l Immunohistochemical stains against HTT (e–h) and against p62 (i–l) demonstrating gradually increasing inclusion densities from BA17, BA7, BA4 to BA9. Brown arrows indicate a subset of the inclusions seen in these micrographs. Original magnifications: e–l: 400×, scale bars of e–l: 50 µm