Fig. 6

In vivo miniscope imaging reveals differential vascular regulation at multiple behavioral states at different time points following TBI injury. A Exemplar color-coded plots of blood flow velocities from control and TBI mice (6 and 17 dpi) under isoflurane anesthesia, awake and running conditions. (Color scale codes the velocity in mm/s). B In TBI mice under anesthesia there is no relationship between microvascular blood velocity to days post injury (R² = 0.0832). C Under running conditions there is a stronger correlation between blood velocity and days after injury (R² = 0.4154). D Vessel diameter and blood flow velocity exhibit a modest relationship irrespective of days post injury (R² = 0.524). E There is a significant temporal decrease in blood velocity in TBI mice (n = 3) that by 10 dpi plateaued but never reaches control mice (n = 4) values (two-way repeated ANOVA group effect (p < 0.0001) with significant differences (p < 0.0001) at 4, 7 and 10 dpi, post-hoc Tukeys) All linear regressions use the full data range to 15 dpi and are plotted as mean and 95% CI