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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Impaired signaling for neuromuscular synaptic maintenance is a feature of Motor Neuron Disease

Fig. 5

Muscle grown from MND patients responded poorly to human motor axons. A Timeline for human derived MNs innervating human skeletal muscle in a microfluidic culture system. B Representative visual fields showing axons of H9 stem cell-derived motor neurons (H9 MNs) immunostained for neurofilament (NF, white arrow). MN axons grew along the microgrooves (white arrow) to enter the muscle chamber. Direction of axonal growth is indicated by the yellow arrow. D Representative visual field from the muscle chamber, showing neurofilament (red) and SV2 (pink) positive H9 motor axons intermingled with muscle cells grown from a non-MND donor. White arrows indicate large AChR clusters (green) that were located close to NF/SV2-positive axons.  E Muscle cells sourced from an MND patient displaying small AChR clusters (green, yellow arrows) adjacent to NF/SV2 positive axons. Muscle cell nuclei in C, D were labelled with Hoechst 33,342 (blue)

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