Fig. 7

Specific Comp expression in DMD FAPs. a T-SNE clustering highlighting FAP population. b Violin plots showing the level of expression of Pdgfrα, Dcn, S100a4, Col8a1, Fabp4, Vim and Comp in WT and DMD diaphragms at 12 months of age. c T-SNE visualisation of Comp expression in WT and DMD FAPs. d Heatmap showing the relative expression of Pdgfrα, S100a4, Fabp4 and Comp in WT and DMD FAPs. e Validation of Comp mRNA expression level in WT and DMD quadriceps at 12 months of age. f Representative immunofluorescence of COMP (red), LAMININ (green) and nuclei (blue) in 12-month-old WT and DMD rat tibialis anterior (scale bar = 20 μm). g Quantification of COMP expression area (f) at 12 months for WT and DMD rats. h Concentration of circulation COMP in sera of WT and R-DMDdel52 rats at 6 and 12 months of age. Values are expressed in ng/ml. i Representative immunofluorescence of COMP (red), LAMININ (green) and nuclei (blue) on control and DMD deltoid biopsies (scale bar = 20 μm). j Quantification the percentage of COMP-positive area on control and DMD human biopsies. k Graphic scheme of COMP expression in DMD fibrotic deposition