Fig. 2
From: Insulin-like growth factor 5 associates with human Aß plaques and promotes cognitive impairment
7-day voluntary wheel running increases Bdnf protein levels in the hippocampus and anterior cortex of wild-type but not of Bp5 tg+ mice. a Running distance per day of 3-month-old wild-type and Bp5 tg+ mice. b Running distance per day of 18-month-old wild-type and Bp5 tg+ mice. c, d, e Bdnf protein levels determined by ELISA in the hippocampus, anterior and posterior cortex of 3-month-old wild-type and Bp5 tg+ mice 7 days after voluntary wheel running. f, g, h Bdnf protein levels determined by ELISA in the hippocampus, anterior and posterior cortex of 18-month-old wild-type and Bp5 tg+ mice 7 days after voluntary wheel running. i Hippocampal sections of 3-month-old sedentary wild-type (WTSed), running wild-type (WTRun), sedentary Bp5 tg+ (Bp5 tg+ Sed) and running Bp5 tg+ (Bp5 tg+ Run) mice stained for Bdnf. Intense Bdnf staining detected in the mossy fiber pathway of WTRun mice compared to WTSed mice. 7-day of voluntary wheel running did not increase Bdnf intensity in the mossy fiber pathway of Bp5 tg+ Run mice. j Higher magnification view of the CA3 region of WTSed, WTRun, Bp5 tg+ Sed, Bp5 tg+ Run sections stained for Bdnf. Note the intense staining in the stratum lucidum of CA3, containing the axon terminals of mossy fibers. k Super resolution microscopy images of a single CA3 neuron labeled with antibodies against Synaptophysin and Bdnf of WTSed, WTRun, Bp5 tg+ Sed and Bp5 tg+ Run sections. A higher Bdnf intensity was found at the presynapse of WTRun compared to WTSed. Bdnf intensity of Bp5 tg+ Run appeared comparable to that of WTSed and Bp5 tg+ Sed mice. Data represents mean ± SD, a, b = unpaired t-test; c, d, e, f, g, h = One-way ANOVA, Tukey’s multiple comparison tests; **p < 0.01, ***p < 0.001; n = number of mice; Scale bars i = 200 µm, j = 50 µm, k = 10 µm