Fig. 3

Mical affects Tau via its redox enzymatic activity. a Survival curves for animals expressing panneuronally the indicated transgenes at 30 °C, in comparison with elav^C155-GAL4/+;tub-Gal80ts/+ control (CN). Statistical analysis indicated significant differences in longevity after accumulation of hTau^0N4R alone and upon co-overexpression with UAS-MicalΔredox mutant transgene. b Response of flies expressing panneuronally the indicated transgenes to the oxidant molecule paraquat. Flies have been treated with 30 mM paraquat and mortality was scored after 28 h. Control flies are driver single copy flies (CN, elav^C155-GAL4/+) and flies that overexpress MicalΔredox. c Endogenous microtubules (MT) and phalloidin-bound F-Actin were isolated from flies expressing panneuronally the indicated transgenes. Pellet (p) and supernatant (s) fractions have been probed for Tau, Tubulin and Actin respectively. The ratio of the relative level of Tau in the supernatant to the Tau in the pellet fractions was used for quantification and shown as ratios of their means ± SEM relative to the ratio of Tau expressed alone, which is arbitrarily set to 1. d Representative Western blot of aqueous soluble and insoluble fractions generated from adult heads following panneuronal expression of the indicated transgenes. Syntaxin (Syx) was used as loading control. The bars represent the mean ± SEM relative sol/ins ratios of Tau upon MicalΔredox co-overexpression, over that of Tau alone