Fig.2

Mical affects Tau toxicity in vivo. a Virgin female flies bearing the hTau^0N4R transgene were crossed with elav^C155-GAL4, elav^C155-GAL4;UAS-Mic/CyO and elav^C155-GAL4;UAS-MicRNAi/CyO males. Bars represent the mean number of non-balancer-bearing progeny females over males ± SEM of the indicated genotypes. w¹¹¹⁸ females were crossed with elav^C155-GAL4 males and the ratio of their progeny female versus male was considered as control progeny (CN). w¹¹¹⁸ females have equally been crossed with elav^C155-GAL4;UAS-Mic/CyO and elav^C155-GAL4;UAS-MicRNAi/CyO males to assess the viability of Mical transgenes in the absence of Tau (right panel). Stars indicate significant difference from CN. b Survival curves for animals expressing panneuronally the indicated transgenes at 30 °C, in comparison with elavC15-GAL4/+;tub-Gal80ts/+ control (CN). Statistical analysis indicated significant differences in longevity after accumulation of hTau^0N4R alone and upon co-overexpression with Mical. c. Response of flies expressing panneuronally the indicated transgenes upon treatment with 30 mM paraquat for 28 h. Stars indicate significant difference from control (CN, elav^C155-GAL4/+). d Memory performance of animals expressing in the adult CNS for 12 days hTau^0N4R alone and upon attenuation of Mical levels. Controls (light grey bars) were the elav^C155-GAL4/+;tub-Gal80ts/+ flies and animals expressing the UAS-Mical RNAi transgene alone. The genotypes of all animals are indicated below each bar. Star indicates significant differences from both controls. The number of experimental replicates (n) is indicated within the bars