Skip to main content

Table 3 Antibodies used for immunohistochemistry (IHC) and western blot (WB)

From: AQP4 labels a subpopulation of white matter-dependent glial radial cells affected by pediatric hydrocephalus, and its expression increased in glial microvesicles released to the cerebrospinal fluid in obstructive hydrocephalus

Antibody

Host

Dilution IHC

Dilution WB

Dilution Flow cytometry

Lot number, manufacturer

GFAP

Rabbit

1:400

1:1000

–

#7260, Abcam

GFAP

Rat

1:200

–

1:100

#ab279291, Abcam

AQP4

Rabbit

1:1500

1:1000

1:100

#PAB 20767, Abnova

S100ß

Rabbit

1:100

–

–

#Ab52642, Abcam

Transthyretin

Rabbit

–

1:1000

–

#A0002, Dako

Vimentin

Rabbit

1:200

–

–

#ab92547, Abcam

Vimentin

Mouse

1:200

–

–

#ab8978, Abcam

Anti-mouse Alexa Fluor 555

Goat

1:300

–

–

#AQ21422, Thermo Fisher Scientific

Anti-rabbit Alexa Fluor 488

Goat

1:300

–

–

#A11034, Thermo Fisher Scientific

Anti-rat FITC

Goat

–

–

1:100

#Ab6840, Abcam

Anti-rabbit APC

Goat

–

–

1:100

#Ab130805, Abcam

Peroxidase-conjugated anti-rabbit

 

–

1:10,000

–

#70742, Cell Signaling Technology