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Table 3 Antibodies used for immunohistochemistry (IHC) and western blot (WB)

From: AQP4 labels a subpopulation of white matter-dependent glial radial cells affected by pediatric hydrocephalus, and its expression increased in glial microvesicles released to the cerebrospinal fluid in obstructive hydrocephalus

Antibody Host Dilution IHC Dilution WB Dilution Flow cytometry Lot number, manufacturer
GFAP Rabbit 1:400 1:1000 #7260, Abcam
GFAP Rat 1:200 1:100 #ab279291, Abcam
AQP4 Rabbit 1:1500 1:1000 1:100 #PAB 20767, Abnova
S100ß Rabbit 1:100 #Ab52642, Abcam
Transthyretin Rabbit 1:1000 #A0002, Dako
Vimentin Rabbit 1:200 #ab92547, Abcam
Vimentin Mouse 1:200 #ab8978, Abcam
Anti-mouse Alexa Fluor 555 Goat 1:300 #AQ21422, Thermo Fisher Scientific
Anti-rabbit Alexa Fluor 488 Goat 1:300 #A11034, Thermo Fisher Scientific
Anti-rat FITC Goat 1:100 #Ab6840, Abcam
Anti-rabbit APC Goat 1:100 #Ab130805, Abcam
Peroxidase-conjugated anti-rabbit   1:10,000 #70742, Cell Signaling Technology