Fig. 4

Comparison of lectin-perfused blood vessels and IHC of all blood vessels (Glut1⁺). A, D Representative fluorescence microscopy images of perfused Lectin-649 signal in lumbar spinal cord sections of control (A) and EAE (D). B, E Representative images of Glut1⁺ blood vessels stained by IHC and imaged with a confocal microscope in control (B) and EAE (E). C, F Representative images of lectin-perfused Glut1⁺ vessels in control (C) and EAE (F). G Quantification of the number of Glut1⁺ vessels in spinal cord sections from EAE and control mice (unpaired t test, n = 5–6 per group, mean ± SEM). H Percentage of Glut1⁺ vessels that were lectin-perfused in spinal cord sections of EAE and control mice (unpaired t test, n = 5–6 per group, mean ± SEM). *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar in A,D 400 μm and in B-C, E–F 40 μm. Abbreviations: Glut1, Glucose transporter type 1; DAPI, 4′,6-diamidino-2-fenilindol