Fig. 2
From: Mapping of neuroinflammation-induced hypoxia in the spinal cord using optoacoustic imaging
Oxygen administration improved oxygenation of the spinal cord in EAE mice. A Diagram depicting the experimental procedure of the oxygen enhancement challenge (OT-OE) using MSOT. B Continuous measurement of oxygen saturation in the spinal cord of EAE and control mice during OE-OT (n = 11–13 per group, mean ± SEM). C MSOT-derived oxygen saturation (SO2MSOT) in the spinal cord of EAE and control mice while breathing medical air (21% oxygen, light blue) and after 15 min breathing 100% oxygen (dark blue) (2-way ANOVA and Tukey post-hoc test, n = 11–13 per group, median, quartiles, min, max). D Difference in oxygen saturation (ΔSO2MSOT) of the spinal cord between breathing air and 100% oxygen in EAE and control mice (unpaired t test, n = 11–13 per group, median, quartiles, min, max) E SO2MSOT in the spinal cord of EAE and control mice while breathing medical air before and after OE-OT (before administering 100% oxygen and after mice stopped breathing 100% oxygen) (2-way ANOVA and Tukey post-hoc test, n = 11–13 per group). F Representative images and schematic depiction of dorsal and ventral lumbar spinal cord division. G MSOT-derived oxygen saturation in the dorsal and ventral spinal cord of EAE and control mice while breathing medical air (light blue) and after 15 min breathing 100% oxygen (dark blue) (2-way ANOVA and Tukey post-hoc test for dorsal and ventral separately, n = 11–13 per group, median, quartiles, min, max). *p < 0.05, **p < 0.01, ***p < 0.001. Abbreviations: OE-OT, oxygen enhancement challenge; ΔSO2MSOT, difference in oxygen saturation between air and oxygen phases; diff, statistical difference