Fig. 4

Lysosome and lysosomal trafficking defects in the cerebellum of aged Tmem106b^−/− mice. a, b Western blot analysis of lysosomal proteins and GAPDH in 16-month-old WT and Tmem106b^−/− cerebellar lysates. Protein levels were quantified and normalized to GAPDH in b. HM: high molecular weight. n = 5, **, p < 0.01, ***, p < 0.001, unpaired t-test. Asterisk indicates non‐specific bands. c, d Immunostaining of AnkG and Cath D in the cerebellar sections from 16‐month‐old WT and Tmem106b^−/− mice. Representative images from the Purkinje cell layer are shown. The percentage of axon initial segments (AISs) with Cath D-positive lysosomal vesicles were quantified in d. n = 3. ****, p < 0.0001, unpaired t-test