Fig. 1

Degeneration of Purkinje cells in aged Tmem106b^−/− mice. a, b Cerebellar sections from 16‐month‐old WT and Tmem106b^−/− mice were stained with anti-calbindin antibody and Hoechst. Purkinje cell numbers in the anterior lobe (AL) and other regions (Others) were quantified in b. Scale bar = 100 µm. n = 3–4, ***, p < 0.001, unpaired t-test. c, d Cerebellar sections from 16‐month‐old WT and Tmem106b^−/− mice were immuno-stained with antibodies of calbindin and parvalbumin (PVALB). Zoom-in images show PVALB-positive interneurons in the MCL adjacent to the region with Purkinje cell loss. The number of PVALB-positive interneurons in the molecular layer of the anterior lobe (AL) was quantified in d. Scale bar = 100 µm. n = 3, ***, p < 0.001, unpaired t-test. e, f Western blot analysis of the protein level of Calbindin, NeuN, and TMEM106B in 16-month-old WT and Tmem106b^−/− cerebellar lysates. GAPDH was used as an internal control. Relative protein levels were quantified in f. n = 5, ***, p < 0.001, unpaired t-test