Fig. 4

RIPK3 inhibition reduces neurodegeneration and memory loss induced by Aβo in mice. A, B Representative images and quantitative analysis of brain sections from wild-type mice at 18 days post-injection, treated as indicated in the images and immunolabeled with an anti-pMLKL antibody (scale bar, 150 μm). C, D Representative micrographs and quantitative measurement of brain sections from wild-type mice at 18 days post-injection, treated as indicated in the images, and stained with Fluoro-Jade C (scale bar, 150 μm). n = 10 per group, 4 sections per brain, one image of the hippocampus per section was analyzed. E, F, H, I Graphs represent quantitative analyzes of the indicated parameters, obtained from the MWM test. G The heat map shows a graphic representation of the time that mice, treated as indicated in the image, spent in the target quadrant (TQ) during the MWM test. J Association analysis of Fluoro-Jade C stained area and cognitive performance was done by linear regression. Data are presented as mean ± S.E.M. Data in B, D-F, H, and I were analyzed by one-way ANOVA followed by Bonferroni post-test. Data in J were analyzed by linear regression using Pearson correlation. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001