Fig. 7
Diseased TgM83+/− mice harbor deposits of pathologic α-synuclein in various brain regions. To reveal deposits of pathologic α-synuclein, coronal tissue sections of different brain regions of diseased TgM83+/− mice collected at 360 days after stroke were stained with the pSyn#64 antibody against α-synuclein phosphorylated at serine 129. Diseased animals that had been subjected to stroke but not sham-treated animals showed cellular deposits of pathologic α-synuclein throughout the cerebrum, including the motor cortex, the striatum, the amygdala, the thalamus, the hypothalamus, the substantia nigra, the superior colliculus, and the pons (a). Deposits of pathologic α-synuclein in the brains of diseased TgM83+/− mice at 360 days after MCAO were quantified as phosphorylated α-synuclein-positive cells per square millimeter from four diseased animals per brain region (Additional file 4), and the data were summarized in heat maps spanning five rostral-to-caudal brain areas (b). Asterisks indicate the stroke side. Scale bars represent 20 µm